条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Front. Behav. Neurosci., 7:17, (2013).<br>研究成果の公表にあたって謝辞の表明を必要とする。 Cre/loxP system ShigeYoshi ITOHARA B (1-3 months) synthetic splice acceptor sequence, P1 phage loxP sites, STOP (mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, polyA), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal, mouse ROSA26 genomic DNA B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito> B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito> Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> B（1〜3か月） true <a href='https://brc.riken.jp/mus/pcr02657'>Genotyping protocol -PCR-</a> Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygote x Homozygote [or Crossing to C57BL/6JJcl] In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17, (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Gt(RSA)26Sor遺伝子座にNLS-lacZをノックインしたレポーターマウス。組織特異的Cre発現マウスとの交配によりlacZを発現する。核がlacZにより可視化され、細胞体の局在が観察可能。ホモマウスは外見上特に異常は認められない。 E14 [129P2/OlaHsd] 理化学研究所脳科学総合研究センター・糸原重美先生。E14 ES細胞由来。C57BL/6Jコンジェニック。 糸原　重美 ROSA nls LacZ ROSA nls LacZ <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/mar_2013_mm" target="_blank">Mouse of the Month Mar 2013</A> PGK-neo cassette flanked by loxP sites and NLS-lacZ were inserted into the Gt(ROSA)26Sor locus. The NLS-lacZ expression can be generated by crossing with tissue-specific Cre mice. The expression is localized within cell nucleus. Homozygous mutant mice show no obvious abnormality. Developed by Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells derived from 129P2/OlaHsd were used. The mutant mice were backcrossed to C57BL/6J. Fluorescent Proteins/lacZ System RBRC02657